Receptor de hormona tiroidea y metamorfosis en anfibios: Nuevas perspectivas con las nuevas tecnologías de secuenciación

El viernes 20 de mayo el Dr. Laurent Sachs, Museo Nacional de Historia Natural, París, Francia, dictará el seminario Receptor de hormona tiroidea y metamorfosis en anfibios: Nuevas perspectivas con las nuevas tecnologías de secuenciación, el que se realizará en el Auditorio S. Mancinelli, a las 11:00 hrs., ubicado en el zócalo edificio del Arco UdeC.

Summary

Thyroid hormones (THs) regulate diverse cellular processes from mitosis to apoptosis, from metabolism to growth and development. One striking developmental processes orchestrated by THs is amphibian metamorphosis. Tadpoles transformation is marked by dramatic TH-induced changes including de novo morphogenesi, tissue remodelling and organ resorption. These changes involve casacdes of gene regulation initiated by THs and their receptors (TR). Our aim is to decipher the molecular mechanisms controlling the cascades of transcriptional programs dependant of THs signalling in the context of metamorphosis. ChIA-PET (chromatin interaction analysis using paired end tag sequencing) was used to build a genome wide profile of the thyroid hormone receptor (TR) binding sites and to map the physical interactions between DNA bound TR complexes. To link genomic TR binding sites and T₃-response genes, the mapping on the genome of the T₃-response genes has been performed using transcriptomic data (RNA-Seq and RNA-PET). These results have highlighted the importance of genome assembly and annotation for functional genomic analysis hat could be strongly improved by next generation sequencing technologies. The first data have also opened new perspective for functional study of pertinent genes in the context of cell fate decision.